Regulatory

Part:BBa_J119440:Design

Designed by: Todd Eckdahl   Group: Eckdahl Lab   (2019-07-18)


c-Myc Promoter G-quadruplex and i-Motif


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

I had to design the sticky ends of each 5' end such that they could each be complementary to an Eco RI cut site.


Source

This part comes from the human genome. It comes from the Nuclease Hypersensitive Element III of the human c-Myc promoter.

References